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Image Search Results


Primary antibodies

Journal: British Journal of Pharmacology

Article Title: Prenatal and postnatal alcohol exposure increases vulnerability to cocaine addiction in adult mice

doi: 10.1111/bph.14901

Figure Lengend Snippet: Primary antibodies

Article Snippet: The Immuno‐related procedures used comply with the recommendations made by the British Journal of Pharmacology (Alexander et al., 2018 ). table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Primary antibody Description Host Dilution Company Catalogue number RRID ΔFosB Delta FosB Mouse 1:250 Abcam #ab11959 AB_298732 CREB cAMP‐response element binding protein Rabbit 1:500 MERCK #04‐767 AB_1586959 D 1 R Dopamine receptor 1 Rabbit 1:500 Abcam #ab20066 AB_445306 D 2 R Dopamine receptor 2 Rabbit 1:500 MERCK #324393 AB_10681601 DARPP‐32 Dopamine‐ and cAMP‐regulated phosphoprotein Rabbit 1:1,000 Cell Signaling #2306 AB_823479 GAPDH Glyceraldehyde‐3‐phosphate dehydrogenase Mouse 1:2,500 Santa Cruz Biotechnology #sc‐365062 AB_10847862 GluA1 AMPA receptor subunit 1 Rabbit 1:1,000 MERCK #ABN241 AB_2721164 GluA2 AMPA receptor subunit 2 Rabbit 1:1,000 MERCK #AB1768‐I AB_2313802 pCREB Phosphorylated CREB Rabbit 1:1,000 MERCK #06‐519 AB_310153 pDARPP‐32 (Thr34) Phosphorylated DARPP‐32 (Thr34) Rabbit 1:1,000 MERCK #ab9206 AB_347689 β‐Tubulin β‐Tubulin Mouse 1:5,000 BD PharMingen #556321 AB_396360 β‐Tubulin Class III β‐tubulin Rabbit 1:1,000 Abcam #ab6046 AB_2210370 Open in a separate window Primary antibodies

Techniques: Binding Assay

GluA1/GluA2 ratio and striatal ΔFosB expression were altered in PLAE mice after cocaine‐primed reinstatement session. (a–f) Western blot analyses of GluA1/GluA2, pCREB/CREB, pDARPP‐32/DARPP‐32, ΔFosB, D1R, and D2R protein expression in the PFC and STR of PLAE and control mice following cocaine‐induced reinstatement. Student's t test, * P < .05 control versus PLAE group (n = 5–6 per group). The numbers in the bars represent the amount of individuals in the group. The lower panels show representative fluorescent immunoblots

Journal: British Journal of Pharmacology

Article Title: Prenatal and postnatal alcohol exposure increases vulnerability to cocaine addiction in adult mice

doi: 10.1111/bph.14901

Figure Lengend Snippet: GluA1/GluA2 ratio and striatal ΔFosB expression were altered in PLAE mice after cocaine‐primed reinstatement session. (a–f) Western blot analyses of GluA1/GluA2, pCREB/CREB, pDARPP‐32/DARPP‐32, ΔFosB, D1R, and D2R protein expression in the PFC and STR of PLAE and control mice following cocaine‐induced reinstatement. Student's t test, * P < .05 control versus PLAE group (n = 5–6 per group). The numbers in the bars represent the amount of individuals in the group. The lower panels show representative fluorescent immunoblots

Article Snippet: The Immuno‐related procedures used comply with the recommendations made by the British Journal of Pharmacology (Alexander et al., 2018 ). table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Primary antibody Description Host Dilution Company Catalogue number RRID ΔFosB Delta FosB Mouse 1:250 Abcam #ab11959 AB_298732 CREB cAMP‐response element binding protein Rabbit 1:500 MERCK #04‐767 AB_1586959 D 1 R Dopamine receptor 1 Rabbit 1:500 Abcam #ab20066 AB_445306 D 2 R Dopamine receptor 2 Rabbit 1:500 MERCK #324393 AB_10681601 DARPP‐32 Dopamine‐ and cAMP‐regulated phosphoprotein Rabbit 1:1,000 Cell Signaling #2306 AB_823479 GAPDH Glyceraldehyde‐3‐phosphate dehydrogenase Mouse 1:2,500 Santa Cruz Biotechnology #sc‐365062 AB_10847862 GluA1 AMPA receptor subunit 1 Rabbit 1:1,000 MERCK #ABN241 AB_2721164 GluA2 AMPA receptor subunit 2 Rabbit 1:1,000 MERCK #AB1768‐I AB_2313802 pCREB Phosphorylated CREB Rabbit 1:1,000 MERCK #06‐519 AB_310153 pDARPP‐32 (Thr34) Phosphorylated DARPP‐32 (Thr34) Rabbit 1:1,000 MERCK #ab9206 AB_347689 β‐Tubulin β‐Tubulin Mouse 1:5,000 BD PharMingen #556321 AB_396360 β‐Tubulin Class III β‐tubulin Rabbit 1:1,000 Abcam #ab6046 AB_2210370 Open in a separate window Primary antibodies

Techniques: Expressing, Western Blot